3T3-J2 Cell Line from Kerafast

Product Specs
Item3T3-J2 Cell Line
CompanyKerafast
Price Pricing Info Get Pricing Get Quote for this Product
Catalog NumberEF3003
Quantity1 vial
TypeCell Line
Cell Type (standardized)Fibroblasts
HostMouse
Cell TypeEmbryonic mouse fibroblasts
Source/Expression System12-13 days mouse embryos

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Kerafast

2 Shaker Road, Unit B101

Shirley, MA 01464

United States

Phone: 1-800-546-1760
1-617-336-3228 (Mon–Fri 8:30AM–5:30PM EST)
Fax: 1-866-727-9826

Email: CustomerService@kerafast.com

Company Profile

Website: www.kerafast.com

(1) L1077P CFTR pathogenic variant function rescue by Elexacaftor-Tezacaftor-Ivacaftor in cystic fibrosis patient-derived air-liquid interface (ALI) cultures and organoids: in vitro guided personalized therapy of non-F508del patientsRespiratory researchSeptember 6, 2023Lo Cicero S, Castelli G, Blaconà G, Bruno SM, Sette G, Pigliucci R, Villella VR, Esposito S, Zollo I, Spadaro F, et al.co‐cultivated with irradiated (30 Gy) murine J2 Swiss 3T3 fibroblasts (Kerafast, Boston, MA, USA, code EF3003) in F medium (3:1 v/v F‐12 Nutrient Mixture Ham (Gibco, code 11765054): DMEM (Gibco, code 31765-027)

(2) Protocol for isolating human BCAM-positive corneal progenitor cells by flow cytometry and cell sortingSTAR ProtocolsSeptember 3, 2023Yuzuru Sasamoto, Philip C. Yeung, Johnathan Tran, Markus H. Frank, Natasha Y. Franklines,Experimental models: Cell lines,Experimental models: Cell lines ,, 3T3-J2 cell line,Kerafast,EF3003 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, BD FACSDiva,BD Biosciences,8.0.1 FlowJo,BD

(3) Improved cytosine base editors generated from TadA variantsNature BiotechnologyMay 1, 2023Dieter K. Lam, Patricia R. Feliciano, Amena Arif, Tanggis Bohnuud, Thomas P. Fernandez, Jason M. Gehrke, Phil Grayson, Kin D. Lee, Manuel A. Ortega, Courtney Sawyer, et al.long-lived PHH cultures involved the additional coculturing of 3T3-J2 murine fibroblasts (Kerafast, EF3003) at 2.0 × 104 cells per well to the established PHH monocultures. PHH cocultures were maintained with

(4) Activation of the NLRP1 inflammasome in human keratinocytes by the dsDNA mimetic poly(dA:dT)Proceedings of the National Academy of SciencesJanuary 31, 2023Jeffrey Y. Zhou, Mrinal K. Sarkar, Ken Okamura, John E. Harris, Johann E. Gudjonsson, Katherine A. Fitzgeraldwithin 16 h of isolation. Cell Culture. Lenti-X 293T (lab stock, Takarabio #632180), 3T3-J2 (Kerafast #EF3003), Hela (lab stock), and VeroE6 (lab stock) cells were cultured in Dulbecco’s Modified Eagle’s Medium

(5) Limbal BCAM expression identifies a proliferative progenitor population capable of holoclone formation and corneal differentiationCell reportsAugust 9, 2022Sasamoto Y, Lee CAA, Wilson BJ, Buerger F, Martin G, Mishra A, Kiritoshi S, Tran J, Gonzalez G, Hildebrandt F, et al.Cell lines,Experimental models: Cell lines,Experimental models: Cell lines 3T3-J2 Cell Line,Kerafast,EF3003 293T cell line,Clontech,632180 Oligonucleotides,Oligonucleotides,Oligonucleotides TaqMan probes,Thermo Fisher Scientific

(6) Mesoporous Silica-Coated Gold Nanoparticles for Multimodal Imaging and Reactive Oxygen Species Sensing of Stem CellsACS Applied Nano MaterialsMarch 25, 2022Chloe Trayford, Darragh Crosbie, Timo Rademakers, Clemens van Blitterswijk, Rudy Nuijts, Stefano Ferrari, Pamela Habibovic, Vanessa LaPointe, Mor Dickman, Sabine van RijtCaCl2, 0.2 ng/mL EGF, and 25 μg/mL bovine pituitary extract. 3T3-J2 cells were purchased from Kerafast (EF3003, Boston, MA). 3T3-J2 cells are a subclone of a mouse embryonic fibroblast line. For 3T3-J2 culture,

(7) Development of Keratinocyte Cell Lines Containing Extrachromosomal Human Papillomavirus GenomesCurrent ProtocolsSeptember 8, 2021Tami L. Coursey, Alison A. McBrideKeratinocyte F-medium 50 mg/ml G418 (Gibco 10131-027) J2 3T3 murine fibroblast feeder cells (Kerafast EF3003) Neomycin resistant J2 3T3 fibroblast feeders (can be generated by transfecting J2 3T3 cells with

(8) Contribution of GATA6 to homeostasis of the human upper pilosebaceous unit and acne pathogenesisNature communicationsOctober 20, 2020Oulès B, Philippeos C, Segal J, Tihy M, Vietri Rudan M, Cujba AM, Grange PA, Quist S, Natsuga K, Deschamps L, et al.HPV16/E6E7 genes32. 3T3-J2 cells were a gift from James Rheinwald and can be purchased from Kerafast (EF3003). Keratinocytes and SebE6E7 sebocyte stocks were maintained on mitotically inactivated 3T3-J2 cells

(9) Protocol for Cloning Epithelial Stem Cell Variants from Human LungSTAR ProtocolsOctober 5, 2020Wei Rao, Suchan Niroula, Shan Wang, Matthew Vincent, Frank McKeon, Wa XianCell Lines,Experimental Models: Cell Lines,Experimental Models: Cell Lines ,, 3T3-J2,Kerafast,Cat# EF3003 ,, Experimental Models: Organisms/Strains,Experimental Models: Organisms/Strains,Experimental Models:

(10) Role of Jagged1-mediated Notch Signaling Activation in the Differentiation and Stratification of the Human Limbal EpitheliumCellsAugust 22, 2020González S, Halabi M, Ju D, Tsai M, Deng SX.density of 300 cells/cm2 on sub-confluent 3T3-J2 mouse fibroblasts (3T3; Kerafast, Boston, MA, USA; Cat # EF3003, RRID: CVCL_W667) that had been growth-arrested with 4 µg/mL of mitomycin C (Sigma-Aldrich, St. Louis,

(11) Generation of esophageal organoids and organotypic raft cultures from human pluripotent stem cellsMethods in Cell BiologyJune 27, 2020Vered Shacham-Silverberg, James M. Wells(S5761, Sigma-Aldrich) Calcium chloride—CaCl2 (C4901, Sigma-Aldrich) Mouse fibroblasts-J2-3T3 cells (EF3003, Kerafast) 10N NaOH (SX0607N-6, Sigma-Aldrich) Transwell 24mm Polyester Membrane Inserts, 6-well

(12) Xenofree generation of limbal stem cells for ocular surface advanced cell therapyStem Cell Research & TherapyDecember 4, 2019Nuria Nieto-Nicolau, Eva M. Martínez-Conesa, Alba M. Velasco-García, Caterina Aloy-Reverté, Anna Vilarrodona, Ricardo P. Casaroli-MaranoCell culture of feeder layers Murine 3T3 Swiss Albino fibroblasts were obtained from Kerafast (3T3-J2, EF3003). Cells were cultured with DMEM 4.5 g/l (Thermo Scientific, MA, USA) supplemented with 10% FCS and 1%

(13) The response of phyllodes tumor of the breast to anticancer therapy: An in vitro and ex�vivo studyOncology LettersSeptember 6, 2019Alicja Urbaniak, Fariba Jousheghany, Youzhong Yuan, Sergio Pi�a‑Oviedo, Adam Huczyński, Magdalena Delgado, Thomas Kieber‑Emmons, Behjatolah Monzavi‑Karbassi, Timothy C. Chambers2 h. Mouse fibroblast conditioned medium was prepared from Swiss 3T3-J2 mouse fibroblasts (cat. no. EF3003, Kerafast, Inc., Boston MA, USA) cultured in DMEM supplemented with 10% bovine calf serum (iron supplemented

(14) LSD1 Inhibition Promotes Epithelial Differentiation through Derepression of Fate-Determining Transcription FactorsCell reportsAugust 20, 2019Egolf S, Aubert Y, Doepner M, Anderson A, Maldonado-Lopez A, Pacella G, Lee J, Ko EK, Zou J, Lan Y, et al.Lines,Experimental Models: Cell Lines,Experimental Models: Cell Lines ,, J2 3T3 Fibroblasts,Kerafast,Cat# EF3003 Phoenix cells-AMPHO,ATCC,Cat# CRL-3213 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, LSD1

(15) Establishment and optimization of epithelial cell cultures from human ectocervix, transformation zone, and endocervix optimization of epithelial cell culturesJournal of Cellular PhysiologyJune 1, 2019Han Deng, Sumona Mondal, Shantanu Sur, Craig D. Woodworthmg/ml), 0.5 ml of 0.1 M NaOH, and various concentrations of mouse 3T3-J2 fibroblasts (Kerafast Cat# EF3003, RRID:CVCL_W667) or human stromal cells. Two ml of this mixture was aliquoted to each well and incubated

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3T3-J2 Cell Line from Kerafast

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3T3-J2 Cell Line from Kerafast

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